Expanded Bilateral Chum Salmon SNP Genetic Baseline for Genetic Stock Identification

The amplicon sequencing chum salmon SNP panel, with 538 polymorphic highly discriminating markers has been developed as part of the first year of this project. A large proportion of the tissue archive was then genotyped using this panel to develop a coastwide baseline for chum salmon. This resulted in a high-resolution, Conservation Unit (CU)-focused genetic baseline for which initial results indicate higher resolution than the previous microsatellite baseline (Sutherland et al., in prep.). The markers included in this panel were bilaterally agreed upon, and calibration of laboratories in the US with Canada has begun. Transferability of mixed-stock analysis between countries will be possible with this panel. However, to date several southern regions within Canada remain difficult to resolve or for other reasons require additional genotyping effort to finalize the southern chum baseline. We propose to augment the SNP baseline in seven specific regions in order to improve genetic stock identification within the south coast, and therefore improve characterization of streams of origin of chum salmon intercepted in fisheries.

S18-SP22 Expanded Bilateral Chum Salmon SNP Genetic Baseline for Genetic Stock Identification (WDFW) 2018 Report